A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling
Amino acid transporters play a vital role in metabolism and nutrient signaling pathways.Typically, transport activity is investigated using single substrates and competing amounts of other amino acids.We used GC-MS and LC-MS for metabolic screening of Xenopus laevis oocytes expressing various human amino acid transporters incubated in complex media